Table 1.
Expression stability values for Neolamarckia cadamba candidate reference genes calculated by BestKeeper.
| Ranking | Tissue | Developmental stage | photosynthetic cycle | Drought treatment | Cold treatment | Total |
|---|---|---|---|---|---|---|
| 1 | ACT7 (1.37 ± 0.36) | PGK (1.83 ± 0.51) | SAMDC (0.35 ± 0.09) | RPL10A (0.61 ± 0.19) | RPS25 (0.67 ± 0.17) | SAMDC (1.43 ± 0.35) |
| 2 | RPL10A (1.43 ± 0.45) | RPL10A (1.91 ± 0.58) | ACT7 (0.64 ± 0.14) | SAMDC (1.04 ± 0.25) | SAMDC (1.35 ± 0.33) | F-BOX (2.71 ± 0.79) |
| 3 | SAMDC (1.53 ± 0.37) | SAMDC (2.1 ± 0.51) | PGK (1.32 ± 0.37) | RPS25 (1.38 ± 0.33) | TUA5 (1.81 ± 0.51) | RPS25 (2.78 ± 0.68) |
| 4 | EF-1-α (1.96 ± 0.58) | F-BOX (2.67 ± 0.78) | RPS25 (1.57 ± 0.39) | ACT1 (1.41 ± 0.38) | Rubisco (1.87 ± 0.41) | PGK (2.85 ± 0.78) |
| 5 | PGK (2 ± 0.56) | ACT7 (2.79 ± 0.68) | ACT1 (1.6 ± 0.41) | ACT11 (1.75 ± 0.46) | RPL10A (2.6 ± A0.79) | RPL10A (3.33 ± 1.01) |
| 6 | F-BOX (2.11 ± 0.62) | TUA5 (2.94 ± 0.75) | TUA5 (1.6 ± 0.41) | F-BOX (1.79 ± 0.52) | ACT11 (2.64 ± 0.64) | TUA5 (3.58 ± 0.96) |
| 7 | TUA5 (2.17 ± 0.6) | TUA2 (3.09 ± 0.76) | RPL10A (1.94 ± 0.55) | TUA5 (1.97 ± 0.53) | F-BOX (2.94 ± 0.87) | ACT11 (3.86 ± 0.98) |
| 8 | ACT11 (2.26 ± 0.57) | RPS25 (3.09 ± 0.76) | TUA2 (2.13 ± 0.49) | TUA4 (2.37 ± 0.59) | TUA4 (3.1 ± 0.77) | ACT1 (4 ± 1.06) |
| 9 | RPS25 (2.46 ± 0.6) | MDH (3.45 ± 0.83) | EF-1-α (2.54 ± 0.61) | PGK (2.52 ± 0.67) | ACT1 (3.26 ± 0.88) | TUA4 (4.41 ± 1.07) |
| 10 | ACT1 (2.54 ± 0.66) | TUA4 (3.52 ± 0.86) | ACT11 (3.11 ± 0.79) | MDH (3.76 ± 0.91) | PGK (3.61 ± 0.99) | MDH (4.94 ± 1.2) |
| 11 | TUA4 (3.22 ± 0.77) | EF-1-α (3.97 ± 1.11) | GAPDH (3.17 ± 0.8) | TUA2 (4.97 ± 1.36) | ACT7 (3.76 ± 0.95) | Rubisco (5.04 ± 1.1) |
| 12 | TUA2 (4.48 ± 1.09) | ACT11 (4.46 ± 1.17) | MDH (4.11 ± 1.02) | EF-1-α (5.12 ± 1.37) | EF-1-α (3.84 ± 1.06) | ACT7 (5.26 ± 1.32) |
| 13 | GAPDH (4.69 ± 1.09) | ACT1 (4.74 ± 1.3) | F-BOX (4.28 ± 1.21) | ACT7 (5.33 ± 1.36) | GAPDH (4.44 ± 1.02) | GAPDH (5.77 ± 1.35) |
| 14 | Rubisco (5.2 ± 1.16) | Rubisco (5.18 ± 1.13) | TUA4 (5.89 ± 1.31) | Rubisco (6.16 ± 1.32) | TUA2 (5.07 ± 1.32) | TUA2 (6.21 ± 1.57) |
| 15 | MDH (5.28 ± 1.24) | GAPDH (5.31 ± 1.21) | Rubisco (7.17 ± 1.56) | GAPDH (6.23 ± 1.48) | MDH (6.25 ± 1.56) | EF-1-α (6.44 ± 1.77) |
Note: Fifteen candidate reference genes are evaluated by the lowest values of the coefficient of variance (CV) and standard deviation (SD). The number in the bracket indicated that CV ± SD.