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Pregnancy and p53 expression. 5-day-pregnant WF rats and age-matched controls (five each) obtained from Sprague–Dawley, Inc. were allowed to deliver and nurse their pups for 7 days, after which the pups were weaned. The mammary glands of the mothers were allowed to involute for 28 days, at which time point they were collected for immunohistochemical staining of p53. (A and B) AMV; (C and D) pregnant-involuted.
