Fig. 1.

After ischemic stroke p-AKT and P-ERK 1/2 levels are decreased. a and b Double-immunofluorescence staining of p-AKT or p-ERK 1/2 with NeuN in the peri-infarct area of cortex 24 h or 72 h after I/R compared with the ipsilateral sham, NeuN performes green, P-AKT and p-ERK 1/2 is shown in red and hochest is shown in blue. Scale bar, 20 µm. c and d Western blots showing a decreasing expression in p-AKT (c) and p-ERK 1/2 (d) at the indicated time points after I/R at rats (left). Right: quantification analysis of normalized p-AKT and p-ERK 1/2 levels (n = 6 per time points, *P < 0.05 vs. the sham). e and f Western blots of p-AKT (e) and p-ERK 1/2 (f) in OGD-treated cultured primary neurons (left) and quantification analysis of p-AKT and p-ERK 1/2 levels (right), showing a similar down-regulation with rats sample (n = 6, *P < 0.05 vs. the control). The data are expressed as mean ± SE. Statistical analysis was implemented by student’s t-test and variance analysis. (Color figure online)