Fig. 4.

BpV(pic) not only through inhibit PTEN lipid phosphatase activity but also independently of PTEN to up-regulation p-ERK 1/2 level. a Western blots analysis of p-ERK 1/2 levels in SH-SY5Y cells treated with bpV(pic) (10–500 nM) on right. Left: quantification analysis of p-ERK 1/2 levels treated with bpV(pic) shows an increased expression of normalized p-ERK 1/2 compare with vehicle group (n = 6 independent cultures, *P < 0.05 vs. the vehicle). b The p-ERK 1/2 levels in SH-SY5Y cells transfected with NsiRNA or siRNApten then treated with bpV(pic) (200 nM) (n = 6 independent cultures, *P < 0.05 vs. the NsiRNA + vehicle). c The levels of p-ERK 1/2 in U251 cells transfected with PTEN-cDNA WT and G129E (n = 6 independent cultures, *P < 0.05 vs. the EV). d The levels of p-ERK 1/2 increased in PTEN-deficient cell U251 cells when treated with bpV(pic) (50–1000 nM). Quantification analysis of p-ERK 1/2 levels on the right (n = 6 independent cultures, *P < 0.05 vs. the vehicle). e the ERK 1/2 levels have no change by bpV(pic) in vitro (n = 6 independent cultures). The data is expressed as mean ± SE. Statistical analysis was implemented by student’s t-test and variance analysis