Fig. 1.

Increased migration into tumors after Treg depletion. CFSE-labelled MLN lymphocytes were transferred into DT treated APC^min/+/DEREG and control APC^min/+ mice. 2 days later MLN, spleen, unaffected small intestine (SI) and intestinal tumors were harvested and migrated CFSE-labelled cells were examined by flow cytometry. a Percentage of CFSE⁺ lymphocytes recovered after adoptive transfer among all lymphocytes in MLN, spleen, small intestine and tumor. b Percentage of CFSE⁺CD4⁺ and CFSE⁺CD8⁺ cells among all CD4⁺ and CD8⁺ cells, respectively, in small intestine and tumors. c Number of CFSE⁺ CD4⁺ and CFSE⁺CD8⁺ T cells per gram tissue recovered after adoptive transfer in small intestine and tumors. CD25 (d) and CD69 (e) expression among CFSE⁺ cells were characterized using flow cytometry both by all migrated CFSE⁺ lymphocytes and by all migrated CD4⁺CFSE⁺ cells. Symbols represent individual mice and lines represent median (n = 6–19). * p < 0.05, ** p < 0.01; Mann–Whitney test