Table 4.

Primer Sequence and the Reaction Condition for Genotyping TNFR2 Polymorphisms.

SNP	Primer Sequence	Annealing temperature	Product size (bp)	Restriction enzyme
rs1061622	Forward:5′-TCCTCCAGCTGTAACGTGG-3′	63 °C	TT:246 bp, 45 bp	Hin1II
	Reverse:5′-GACAGGCAGACAGAAGGAGT-3′		GG:291 bp
			TG:291 bp, 246 bp, 45 bp
rs1061624	Forward:5′-TGGGCCAAGTTCCTCTAGTG-3′	58 °C	GG:71 bp, 106 bp	MspA1I
	Reverse:5′-CAGGTCACAGAGAGTCAGGG-3′		AA:177 bp
			GA:177 bp, 71 bp, 106 bp