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. 2018 Jun 18;8:9300. doi: 10.1038/s41598-018-27483-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of Thogoto virus (THOV) antibodies in serum samples from mice using immunofluorescence assay. Sera collected from a mouse infested with EMEM-injected ticks (a) (1:200, No.1); mouse inoculated with 10,000 ffu of THOV (b) (1:3200, No.1); mouse infested with THOV-injected ticks (c) (1:3200, No.6) reacting with THOV-infected baby hamster kidney BHK-21 cells. Arrowheads denote THOV ffu detected by THOV antibodies (red) (bar = 20 μm).