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THOV transmission from ticks injected with THOV through anal pore microinjection to mice.
| Treatment | Viral RNA in liver and spleen* | Seroconversion (%)(positive/total) |
|---|---|---|
| Medium-injected ticks | −(0/3) | 0 (0/5) |
| THOV-infected ticks | +(10/10) | 100 (12/12) |
| THOV-inoculated mice | +(3/3) | 100 (5/5) |
*Real-time PCR was used to detect viral RNA as represented by presence (+) or absence (−).
