Fig. 7. AntagomiR-214 ameliorates liver fibrosis in CCl₄-treated mice by targeting Sufu.

a Schema of the mice experimental model. b RT-qPCR data for miR-214 expression in livers from olive oil-treated, CCl₄/NC-miR-treated, CCl₄/antagomir-214-treated mice (n = 5 per group). c H&E and Masson’s trichrome staining in liver sections from representative mice from each group at ×100 magnification and d Masson-stained fibrosis areas were quantified using the ImagePro Plus software. e RT-qPCR analysis of COL1α1 expression in the livers from mice of each group. f Representative immunohistochemistry images of α-SMA expression in the mouse livers from each group at ×100 magnification. g Western blot analysis for SUFU and internal control in the livers of representative mice from each group and h cumulative densitometric analyses of SUFU expression using Western blotting. Relative expression levels are shown as the means ± s.e.m. obtained from triplicate experiments (unpaired two-sample Student’s t test, *P < 0.05 and **P < 0.01)