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. 2018 Jun 18;8:9293. doi: 10.1038/s41598-018-27574-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The presence of DCs and RCs assessed in the TL and BL of the renografin purified E. chaffeensis fractions. (A) Western blot analysis performed with ClpB polyclonal antibody which revealed higher levels of the protein expression in the BL derived bacterial fraction proteins; (B) cell-free E. chaffeensis recovered from TL and BL assessed for reinfection of naïve Vero cells; infection was detected only with the fraction derived from the TL (B1), but not in the BL (B2) (C). Infectivity of fractionated Ehrlichia organisms from the TL and BL was further confirmed by measuring the numbers of infected cells following incubation for three days following inoculation into naïve HL60 cultures. Infectivity with TL-derived Ehrlichia was significantly more than BL derived bacteria.