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. 2018 Jun 18;8:9307. doi: 10.1038/s41598-018-27638-0

Figure 4.

Figure 4

Effect of isocryptotanshinone on expression of cell cycle- and apoptosis-associated proteins in SGC-7901 and MKN-45 cells. SGC-7901 (A) and MKN-45 (B) cells were serum-starved overnight and treated with the indicated concentration of ICTS and serum for 24 hours, and the expression of Cyclin D1, pRb, Rb, and E2F1 was detected using the Western blot analysis. SGC-7901 (C) and MKN-45 (D) cells were exposed to the indicated concentration of ICTS for 24 hours, and the expression of Mcl-1, Bcl-2, and Survivin was assessed using the Western blot assay. β-actin was used as the loading control. ICTS, isocryptotanshinone.