Fig. 2.

Internal BRAF deletion. a The genomic footprint of BRAF is depicted with exons represented by gray, green, and orange vertical lines. Green and orange exons encode the kinase domain and conserved region 1, respectively. Horizontal lines above exons demarcate rearrangements (blue: tandem duplication; red: deletion). b Outline of wild-type transcript. c Outline of fusion transcript with cDNA sequence of rearrangements (sense orientation) with translation. d Intragenic copy number of BRAF (x-axis: genomic coordinate; y-axis: copy number derived from coverage). e Representative phospho-ERK immunohistochemistry