Table 2.

Antibacterial activities and FabF binding experiments with compounds 1–4

	MIC (μg mL−1)		Binding assay with E. coli FabF C163Q
	S. aureus	K. rhizophila	ka (M−1 s−1)	kd (s−1)	KD (M)
thioPTM (1)	4	1	(3.0 ± 0.1)×105	(1.3 ± 0.1)×10−3	(4.4 ± 0.3)×10−9
thioPTN (2)	1	0.5	(2.3 ± 1.2)×105	(6.9 ± 1.7)×10−3	(3.3 ± 0.9)×10−8
PTM (3)	0.5	1	(5.8 ± 0.9)×105	(5.1 ± 0.9)×10−3	(8.8 ± 1.4)×10−9
PTN (4)	0.25	0.25	(6.2 ± 2.2)×105	(4.5 ± 2.6)×10−2	(7.0 ± 1.7)×10−8

Minimum inhibitory concentrations (MICs) were determined in triplicate using the broth dilution method. Binding assays are reported as means with standard deviations of at least two replicates