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. 2018 Jun 18;9(7):722. doi: 10.1038/s41419-018-0759-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The expression of SNHG7 in CRC cell lines transfection with pcDNA/SNHG7 or pcDNA/Control was analyzed by qRT-PCR. b The proliferative ability of SW480 and HCT-116 cells transfected with pcDNA/SNHG7 or pcDNA/Control was performed by CCK-8 assay. Colony formation (c) and EdU assay (d) were performed in SW480 and HCT-116 cells transfected with pcDNA/SNHG7 or pcDNA/Control. Scale bars = 50 μm. e The expression of cleaved PARP, cleaved caspase-7, and cleaved caspase-3 was analyzed by western blot. f The apoptotic rates of cells were detected by flow cytometry. g The cell cycle progression of SW480 and HCT-116 cells was evaluated after transfection with pcDNA/SNHG7 or pcDNA/Control using Flow cytometry. h Wound scratch assay in pcDNA/SNHG7 or pcDNA/Control transfected SW480 and HCT-116 cells was shown. Scale bars = 50 μm. i Transwell invasion assay in pcDNA/SNHG7 or pcDNA/Control transfected SW480 and HCT-116 cells was shown. Scale bars = 20 μm. The error bars in all graphs represented SD, and each experiment was repeated three times. *p < 0.05