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. 2018 Jun 18;9(7):722. doi: 10.1038/s41419-018-0759-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The differential expression of GALNT1 in CRC tissues and adjacent normal colon tissues was examined. Expressional levels of GALNT1 in CRC cell lines and FHC cell. b Pearson’s correlation curve identified positive correlation between SNHG7 and GALNT1 in CRC tissues. c The expressional levels of GALNT1 mRNA and protein, E-cadherin and Vimentin proteins in CRC cells transfected with siSNHG7 were evaluated by qRT-PCR and western blot. d The levels of GALNT1 mRNA and protein, E-cadherin and Vimentin proteins in CRC cells transfected with pcDNA/SNHG7 were evaluated by qRT-PCR and western blot. e The predicted binding sites of miR-216b to the SNHG7 sequence were shown. f The differential expression of miR-216b in CRC tissues and adjacent normal colon tissues was analyzed. g The levels of miR-216b was investigated in CRC cell lines and human normal colon cell line by qRT-PCR. h Pearson’s correlation curve revealed the negative relevance between SNHG7 and miR-216b expression. i RNA-IP was performed in SW620 and SW480 cells transfected with NC mimic and miR-216b mimic. SNHG7 expression was detected using qRT-PCR. j Luciferase activity of 293T cells cotransfected with miR-216b mimic and luciferase reporters containing SNHG7-Wt or SNHG7-Mut transcript were analyzed. k The predicted binding sites of miR-216b to the GALNT1 sequence were shown. l Pearson’s correlation curve revealed the negative relevance between GALNT1 and miR-216b levels. m Luciferase activity of 293T cells cotransfected with miR-216b mimic and luciferase reporters containing GALLNT1-Wt or GALNT1-Mut transcript were performed. n The levels of GALNT1 transfected with miR-216b inhibitor or siSNHG7 in SW620 cell were analyzed by qRT-PCR and western blot. o The levels of GALNT1 transfected with miR-216b mimic or pcDNA/SNHG7 in SW480 cells were analyzed by qRT-PCR and western blot. The error bars in all graphs represented SD, and each experiment was repeated three times. *p < 0.05