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. 2018 Apr 19;43(8):1779–1788. doi: 10.1038/s41386-018-0073-1

Fig. 5.

Fig. 5

MS-275 treatment of Shank3-deficient mice restores actin filaments at synaptic membrane and increases the transcription of actin regulators. a, b Immunoblots and quantification (mean ± SEM) showing actin in the total lysates, triton-soluble synaptic cytosolic fraction (G-actin) and triton-insoluble synaptic membrane fraction (F-actin) in PFC slices from WT mice or Shank3+/ΔC mice treated with MS-275 (5 mg/kg, i.p., 3x) or saline. *p < 0.05, one-way ANOVA. c, d Quantitative RT-PCR data on the mRNA level of actin regulators in PFC slices from WT mice or Shank3+/ΔC mice treated with MS-275 (5 mg/kg, i.p., 3x) or saline. *p < 0.05, one-way ANOVA. e PCR image showing the input (total DNA), ChIP (AcetyH3-occupied DNA), and no-template control (NTC) signals with a pair of primer designed against the promoter regions of βPIX. f ChIP assay data showing the acetylated H3 level at βPIX promoter in PFC lysates from saline-injected WT, saline-injected Shank3+/ΔC, and MS-275-treated Shank3+/ΔC mice. **p < 0.01, one-way ANOVA