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. 2018 Jun 18;8:9316. doi: 10.1038/s41598-018-27690-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Laser-induced eKR2 photocurrents and flash photolysis with recombinant KR2. Comparison of results from different ionic and pH conditions (a) 110 mM [Na⁺]_i/e at pH 7.2 (mean ± SD, n = 3), (b) 1 mM [Na⁺]_i/e at pH_i/e 7.2 (mean ± SD, n = 4), and (c) 1 mM [Na⁺]_i/e at pH 9.0 (mean ± SD, n = 5). Top: Averaged normalized single turnover eKR2 photocurrents (0 mV) induced by a 7-ns laser flash (525 nm) and corresponding components of the fit of the current decay with a tri-exponential function; inset: pie chart with integrals of the three exponential decay components. Middle: contour plots from flash photolysis measurements of recombinant KR2 under the same conditions together with time traces at the indicated wavelengths. Bottom: time constants resulting from exponential fitting (decay) of the indicated time trace; points represent SVD filtered raw data, line is only guidance to the eye.