. 2018 Apr 9;15:24–29. doi: 10.1016/j.bdq.2018.03.002

Table 2.

Copy number variation of four endogenous references among three non-GE canola cultivars.

Canola cultivar (DNA source)	HMG-I/Y copy numbers^X,Y	FatA(A) Copy numbers^X,Y	CruA copy numbers^X,Y	Ccf copy numbers^X,Y	Comparison of reference means for each cultivar^Z
Legend	6350 ± 217^c	6083 ± 295^b	11885 ± 462^b	11852 ± 186^b	[b, b, a, a]
Eagle	7283 ± 374^b	6145 ± 192^b	13369 ± 356^a	13584 ± 460^a	[b, b, a, a]
Parkland	8444 ± 149^a	11764 ± 284^a	13084 ± 552^a	13397 ± 447^a	[c, b, a, a]

10 ng DNA extracted with Fast ID DNA extraction method was used for ddPCR.

^X Average of four ddPCR measurements plus minus standard deviation.

^Y For each reference gene and copy numbers for the three cultivars, means assigned the same letter vertically are not significantly different (α = 0.05).

^Z For each cultivar, reference means assigned the same letter horizontally are not significantly different (α = 0.05). For example, for Legend, means of HMG-I/Y, FatA(A), CruA and Ccf have the letters ‘b’, ‘b’, ‘a’ and ‘a’, respectively – means of CruA and Ccf were significantly higher than that of HMG-I/Y and FatA(A).