. 2018 Apr 9;15:24–29. doi: 10.1016/j.bdq.2018.03.002

Table 3.

Effect of four DNA extraction methods on the assessment of reference gene copy numbers.

DNA extraction method and cultivar	HMG-I/Y copy numbers	FatA(A) copy numbers	CruA copy numbers	Ccf copy numbers
FID – Legend	6841 ± 149^a	6463 ± 43^a	13741 ± 97^a	13704 ± 304^a
GMQ2 – Legend	4746 ± 234^b	4662 ± 60^bc	9641 ± 288^b	9898 ± 252^b
DMF – Legend	4673 ± 116^b	4801 ± 31^b	9221 ± 106^b	9221 ± 276^c
NSF – Legend	4738 ± 54^b	4548 ± 127^c	9198 ± 289^b	9684 ± 137^bc
FID – Eagle	7018 ± 202^a	6550 ± 137^a	13943 ± 578^a	14417 ± 249^a
GMQ2 – Eagle	4866 ± 89^b	4610 ± 78^b	9730 ± 193^bc	9989 ± 246^b
DMF – Eagle	4487 ± 228^b	4526 ± 5^b	8649 ± 296^c	9009 ± 277^c
NSF – Eagle	5018 ± 525^b	4641 ± 93^b	9963 ± 222^bc	10560 ± 392^b

FID = Fast ID DNA extraction kit; GMQ2 = GM Quicker II DNA extraction kit; DMF = DNeasy® mericon Food kit; NSF = NucleoSpin Food kit. Average of three ddPCR measurements plus minus standard deviation. For each reference gene/cultivar and four DNA extraction methods (vertically), means assigned the same letter are not significantly different (α = 0.05). 10 ng DNA was used for ddPCR.