Figure 4.
Propofol treatment inhibits the growth of xenograft tumors in nude mice. LN299 cells were injected subcutaneously into the right dorsal flank of nude mice. When the average tumor volume had reached ~100 mm3, the mice were randomly divided to two groups (n=6/group) and treated via intraperitoneal injection with propofol (50 mg/kg) or soybean oil (once each day for 20 days). (A) Tumor growth and (B) body weights were measured at the indicated times. *P<0.05. (C) Propofol treatment inhibited LN299 tumor weights. Data are shown as the mean ± standard deviation (n=6). (D) mRNA expression levels of AXIN2, CCND1 and BCL2 in LN299 xenografts were quantified using reverse transcription-quantitative polymerase chain reaction analysis. The mRNA expression was normalized to GAPDH. (E) Propofol treatment inhibited the protein expression of total β-catenin, CCND1 and BCL2 in LN299 xenografts. Tubulin was used as a loading control. CCND1, cyclin D1; BCL2, B-cell lymphoma 2.