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. 2018 May 4;16(1):137–144. doi: 10.3892/ol.2018.8638

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Recombination of pGL3-DKK1 fragments and pGL3-SV40, and measurement of their activity. (A) Verification of reconstructed plasmid by restriction enzyme digestion. (B) Luciferase activity of DKK1-luciferase constructs in A549, H460, H446 and 293 cell lines. *P<0.05 vs. pGL3-b DKK1, dickkopf 1; M, marker.