Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 4;16(1):137–144. doi: 10.3892/ol.2018.8638

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — Recombination of pGL3-DKK1-5-TREs. (A) The location and order of TREs, HS2, DKK1-5 and luciferase in the constructs. The candidate TREs were cloned in front of a DKK1-5 promoter-driven luciferase reporter pGL3-basic vector. (B) Identification of reconstructed TRE reporter plasmids by restriction enzyme digestion. The position on the 1.0% agarose gel corresponds to the specific TRE, as indicated. DKK1, dickkopf 1; TRE, transcriptional regulatory element; HS2, DNase I hypersensitive site II; Poly-A, polyadenylated tail; LUC, luciferase.