Table I.
DKK1 distal promoter fragment primers.
| Promoter fragment | Length (bp) | Primer sequences | Restriction enzymes |
|---|---|---|---|
| DKK1-9 (−938-40) | 978 | F: TCTCCACATTAGCCCACCAC | HindIII/XhoI |
| R: CTGCGGTCCCAGAGTCCT | |||
| DKK1-5 (533–40) | 573 | F: ACTGCGACTCTAAAGGGTTAATG | HindIII/XhoI |
| R: CTGCGGTCCCAGAGTCCT | |||
| DKK1-3 (−350-40) | 390 | F: CCCCTCGGCTCTGTAAAGTAT | |
| R: TGCGGTCCCAGAGTCCT | |||
| DKK1-2 (−282-40) | 320 | F: CAAGTTCCCAGAGTTCCTGCT | HindIII/XhoI |
| R: CTGCGGTCCCAGAGTCCT | |||
| SV40 | 420 | F: CGCAGCACCATGGCCTGA | BgIII/Xho I |
| R: TTGCAAAAGCCTAGGCCTCCA |
DKK1, dickkopf 1; F, forward; R, reverse.