Figure 1.

Expression of YAP is increased in HCC. (A) Representative western blot analysis of YAP protein in HCC (T1-T4) and paired normal tissues (N1-N4) from 4 patients. The expression of β-actin used as a loading control to normalize the YAP protein levels in each sample. (B) Determination of YAP mRNA level in HCC tissues and paired normal tissues by reverse transcription-quantitative polymerase chain reaction. **P<0.01. (C) Expression of YAP was analyzed by immunohistochemistry in HCC of different differentiation degrees using an anti-YAP antibody. Scale bars of the left-hand panels, 200 µm; scale bars of the right-hand panels, 100 µm. YAP, Yes-associated protein; HCC, hepatocellular carcinoma.