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. 2018 May 4;16(1):585–593. doi: 10.3892/ol.2018.8633

Figure 2.

Figure 2.

YAP knockdown inhibits hepatocellular carcinoma cell proliferation. (A) YAP expression is determined in a normal liver cell line and a HCC cell line by western blot analysis. (B) YAP expression in SMMC-7721 cells following transfection with control siRNA (control), YAP siRNA (siYAP) or YAP siRNA and YAP cDNA (Rescue) was measured by western blot analysis. β-actin served as a loading control. (C and D) The effect of YAP siRNA on cell growth. SMMC-7721 cells transiently transfected with control siRNA, YAP siRNA or YAP siRNA and YAP cDNA were cultured for 72 h. (C) Cell viability was measured using an MTT assay and (D) cell proliferation was measured with a BrdU assay. Scale bar, 75 µm. (E) Flow cytometry analysis of SMMC-7721 cells. (F) Western blot analysis of c-myc, p21, caspase 3 and Bax protein expression levels in transfected cells. *P<0.05; **P<0.01. YAP, Yes-associated protein; siRNA/si, small interferring RNA; siYAP, siRNA against YAP; Bax, BCL2 associated X apoptosis regulator.