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. 2018 May 3;16(1):317–325. doi: 10.3892/ol.2018.8624

Figure 1.

Figure 1.

HPV16 E7 promotes the proliferation of breast cancer cells in vitro and in vivo. (A) The expression of HPV16 E7 in MCF-7/HPV16 E7 and MCF-7/vector cells. (B) Cell proliferation as assessed using a MTT assay. (C) Representative images and (D) quantification of colony formation. (E) Representative images and (F) quantification of soft agar colony formation. (G) Tumor volume in mice injected with MCF-7/HPV16 E7 and MCF-7/vector cells (n=6/group). Tumor volume was evaluated at indicated days. (H) Tumor sections were stained with hematoxylin and eosin or with anti-ki-67 to evaluate proliferation, or subjected to IHC staining using an antibody against ki-67. (I) Quantification of ki-67 immunopositive cells in mice injected with MCF-7/HPV16 E7 and MCF-7/vector cells. **P<0.01. HPV, human papilloma virus; OD, optical density; H&E, hematoxylin and eosin.