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. 2018 May 3;16(1):317–325. doi: 10.3892/ol.2018.8624

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — HPV16 E7 promotes the proliferation of breast cancer cells by upregulating COX-2. (A) The expression of COX-2 in MCF-7/HPV16 E7 and MCF-7/vector cells using western blot analysis. (B) The expression of HPV16 E7 and COX-2 in response to siCOX-2 or celecoxib treatment using western blot analysis. (C) Analysis of cell proliferation using an MTT assay. (D) Representative images and (E) quantification of colony formation. (F) Representative images and (G) quantification of soft agar colony formation. *P<0.05, **P<0.01. HPV, human papilloma virus; COX-2, cyclooxygenase-2; siCOX-2, COX-2-specific small interfering RNA.