Figure 2.
Expression of total and mutant BRAF is increased in A375 compared with HEL293A and WM115 cells. (A) Measurement of mRNA level of mutant BRAFV600E in melanoma A375, WM115 and HEK293A cell lines. (B) Measurement of the protein expression of BRAF, pERK1/2, pAKT(S473), pS6 in A375 and WM115 melanoma cell lines by western blot analysis. (C) Cell viability of A375 and WM115 cells, measured using a CCK-8 assay following treatment with 5 nM of siMB3 for 72 h. p, phosphorylated; ERK, extracellular signal-regulated kinase; AKT, RAC-α serine-threonine-protein kinase; S6, S6 ribosomal protein.