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Basic transduction condition. HepG2 cell viability in transduction media in the absence and presence of serum at 6 and 24 h, as the polybrene concentration increases. Data are presented as the mean ± standard deviation, and each group consists of a technical triplicate for 3 biological replicates. a, P<0.05 vs. TM 10% FBS 6 h; b, P<0.05 vs. TM 10% FBS 24 h; c, P<0.05 vs. TM without FBS 6 h and d, P<0.05 vs. TM without FBS 24 h. TM, transduction media; FBS, fetal bovine serum.
