Figure 3.

OCT4 knockdown increases apoptosis in MCF-7 cells. Apoptosis analysis of MCF-7 cells by flow cytometry using Annexin V-fluorescein isothiocyanate and PI double staining following OCT4 knockdown. Apoptotic data were calculated as the percentage change compared with the control. Error bars represent the standard deviation of three replicates. *P<0.05. **P<0.01. LL, normal, live cells without staining; UL, dead cells with PI staining; UR, late apoptotic cells with both Annexin V and PI staining; LR, early apoptotic cells with Annexin V staining; OCT4, octamer-binding transcription factor 4; PI, propidium iodide.