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. 2018 May 2;16(1):522–528. doi: 10.3892/ol.2018.8607

Figure 4.

Figure 4.

Comparison of OCT4B and OCT4A in the regulation of gene expression. (A) Reverse transcription-qPCR analysis of the mutual regulation between OCT4B and OCT4A in MCF-7 cells. Overexpression of OCT4A led to increased expression of OCT4B and vice versa. (B) Western blot analysis of the mutual regulation between OCT4B and OCT4A in MCF-7 cells. (C) qPCR transcriptional analysis of genes involved in cell cycle arrest and apoptosis following OCT4A and OCT4B overexpression in MCF-7 cells. Of note, cyclin-dependent kinase inhibitor 2A codes for two proteins: p16 (or p16INK4a) and p14ARF. Error bars represent the standard deviation from three replicates. *P<0.05. **P<0.01. OCT4, octamer-binding transcription factor 4; Bcl, B-cell lymphoma; Bax, Bcl-2-associated X protein; qPCR, quantitative polymerase chain reaction; N1, exogenous transcription of empty vector; N1-4B, overexpression vector for OCT4B; Red-4A, overexpression vector for OCT4A; NT, negative control; N1-4Bs, overexpression vector for OCT4B; N1-4As, overexpression vector for OCT4A.