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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Chem Res Toxicol. 2017 Nov 28;30(12):2218–2228. doi: 10.1021/acs.chemrestox.7b00288

Figure 3.

Figure 3

Immuno MALDI demonstrating specific capture of diethoxyphospho-tyrosine modified peptide by antibody depY. A) Peptides before incubation with immobilized antibody. Dichlorvos-modified peptide GGYR has masses at 560.2 Da for the dimethoxyphospho adduct and 546.2 Da for the aged monomethoxyphospho adduct. Chlorpyrifos oxon modified peptide YGGFL has masses at 692.2 Da for the diethoxyphospho adduct on YGGFL and 714.2 Da for the sodiated ion. B) Peptides captured by the antibody were identified as the diethoxyphospho adduct on YGGFL. No ions for the dichlorvos-modified peptide were observed. C) Negative control beads (no antibody) captured no diethoxyphospho-Tyr modified peptides.