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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Chem Res Toxicol. 2017 Nov 28;30(12):2218–2228. doi: 10.1021/acs.chemrestox.7b00288

Figure 6.

Figure 6

Binding affinity of depY monoclonal antibody determined by biolayer interferometry using an OctetRED96 instrument. A) Binding and dissociation of porcine tubulin-OP modified on tyrosine by diethoxyphosphate. B) No binding to control porcine tubulin. The depY monoclonal antibody was captured on an anti-mouse IgG biosensor. A concentration series of 100, 50, 25, 12.5 and 6.25 nM porcine tubulin-OP was used to determine the association and dissociation rate constants and the binding affinity for depY. The data points are represented by wavy lines and the fitted data are shown as solid lines.