Animals received a single oral dose of PhIP (100 or 200 mg/kg) and samples were obtained 8 or 24 h later. Striatal dopamine (DA) (A), 3,4-dihydroxyphenylacetic acid (DOPAC) (B) and homovanillic acid (HVA) (C) levels were measured using HPLC with electrochemical detection. Neurotransmitter levels were normalized to total tissue protein and expressed as ng neurotransmitter/mg protein. Dopamine turnover (D) was determined as (DOPAC + HVA)/DA. *p< 0.05, **p< 0.01, ***p<0.001 vs. control; ##p<0.01 8 h vs. 24 h (200 mg/kg); Kruskal-Wallis test, followed by Dunn’s multiple comparisons post hoc test. The data are presented as the mean ± SEM (n = 8–10/treatment).