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Sildenafil-associated decrease in proliferation and vascular cell densities. Immunohistochemical detection of A,B) Ki-67 and C,D) von Willebrand factor in decalcified, paraffin sections of control and sildenafil-treated animals. E, F) Percent vascular density and proliferating cell population were measured using a ratio of von Willebrand factor+ or Ki-67+ cells to total number of hematoxylin-stained nuclei in 5 random fields each at 200× magnification.
