Fig. 2.

PARP1 inhibition reduces proliferation of ovarian cancer cells. (A) Cell cycle distribution of A2780, HO8910 and HEY cells after being treated with PJ-34 for 48 h. The cell cycle distribution was measured by flow cytometry. (B) Colony-forming efficiency of ovarian cancer cells after treatment with PJ-34 for two weeks. (C) Colony-forming efficiency of FTE-187 after treated by different concentration of PJ-34 for two weeks. (D) Colony-forming efficiency of A2780 shPARP1 cells and A2780 shNeg cells. (E) RNAi efficiency was evaluated by Western blotting. The numbers shown below bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to β-actin. Each experiment was repeated three times.*p< 0.05, **p< 0.01 (compared to control, t-test).