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Cryopreservation of samples. The figure shows how liver biopsies in vials containing cryopreservation media were transferred first to a cooler with ice (approximately 2–4 °C), then exposed to nitrogen vapors at the neck tube of a liquid nitrogen tank (−110 °C) and finally were submerged in liquid nitrogen (−196 °C), in order to achieve a gradual and slow freezing of the samples. After this procedure samples were ready for transport and storage at − 80 °C.
