Fig. 2.

Evaluation of mitochondrial function with specific substrates for complex I or II. Respiration rates were measured at 37 °C. Fresh samples are shown in grey, cryopreserved samples in black and frozen samples in green. (A) Oxygen consumption rates were measured after the sequential addition of 10 mM glutamate and 5 mM malate (Glu/Mal), 4 μM ADP, 2 μM oligomycin (Oligo), up to 4 μM FCCP and 0.5 µM rotenone (Rot). (B) Oxygen consumption rates were measured after addition of 20 mM succinate (Succ), 4 μM ADP, 2 μM oligomycin (Oligo) 4 μM FCCP and 2.5 µM antimycin A (Ant A).