Fig. 4.

Assessment of mitochondrial outer membrane integrity by addition of cytochromec. State 3 respiration dependent on complex I (A) and II (B) substrates obtained as described in Fig. 2A and B respectively. The black bars represent state 3 respiration for fresh samples, grey bars show state 3 respiration for cryopreserved samples without the addition cytochrome c and white bars represent state 3 respiration with the addition of 10 µM cytochrome c. Unpaired t-tests were performed using GraphPad Prism v. 6.0 (GraphPad, La Jolla, CA). Significance was set at P < 0.05 for all analyses, ns stands for not significant (n = 12).