Table 2.

Complex II-dependent respiratory parameters.

Species	Storage conditions	State 4 respirationa	State 3 respirationa	Oligomycin resistant respirationa	Oligomycin sensitive respirationa	Maximum respiratory ratea	Non-mitochondrial oxygen consumption ratea	Spare respiratory capacitya	Respiratory control ratio	Coupling efficiency
Cow	Fresh	22 ± 9	55 ± 7	22 ± 5	8 ± 2	86 ± 9	5 ± 2	40 ± 7	3.6 ± 0.6	0.5 ± 0.05
	Cryopreserved	24 ± 9	58 ± 4	41 ± 17*	9 ± 1	75 ± 9	6 ± 2	33 ± 4	2.2 ± 0.2**	0.23 ± 0.02***
	Frozen	17 ± 2	22 ± 4*	34 ± 10*	5 ± 1	41 ± 4*	8 ± 2*	24 ± 5	1.5 ± 0.1****	0.2 ± 0.04***
Rat	Fresh	14 ± 2	50 ± 9	–	–	–	6 ± 2	–	3 ± 0.5	–
	Cryopreserved	17 ± 5*	40 ± 5	–	–	–	8 ± 2	–	2 ± 0.2*	–

The different respiratory parameters were obtained from oxygen consumption rate measurements performed as described in Fig. 2B. All results are expressed as mean ± standard error. Unpaired t-tests were performed using GraphPad Prism v. 6.0 (GraphPad, La Jolla, CA). Significance was set at P < 0.05 for all analyses.

^*P < 0.05.

^**P < 0.01.

^***P < 0.001.

^****P < 0.0001 indicate significant differences between fresh samples and treatments (n = 4–6).

^aRespiratory parameters are expressed as pmol O₂ min⁻¹ mg⁻¹.