Fig. 8.

MiR21 increase causes kidney inflammation. A. mRNA expression of IL-1β, TNFα, and IFN-γ in kidney tissue from mice fed with high-fat diet. NAFLD serve as a control, NAFLD mice exposed to BDCM (NAFLD + BDCM), miRNA 21 gene-deficient mice exposed to BDCM (miR21 KO + BDCM) as assessed by quantitative real-time PCR, the expressions were normalized with 18S and compared to the NAFLD group (*P < 0.05). B. Immunoreactivity of IL-1β as shown by immunohistochemistry in kidney slices from. of NAFLD, NAFLD + BDCM, miR21 KO, and miR21 KO + BDCM group of mice Images were taken at 20×. C. Morphometric analysis of IL-1β immunoreactivity in NAFLD, NAFLD + BDCM, miR21 KO, and miR21 KO + BDCM group of mice (*P < 0.05).