Fig. 2.

Primary neuronal culture. Briefly, pups can be obtained from mice or rats ranging from E18 to p0–3 in age. Desired brain region can then be micro-dissected from the brain. Tissue is then digested using a papain solution for 20 min at 37 °C with gentle agitation every ~3 min. Cells are then triturated using 1 mL pipet tip with smooth edges. Remaining debris should be removed and DNase added. Cells are then centrifuged at ~250×g for 5 min. Cells are then to be resuspended, counted, and plated.