Fig. 3.

Assessing autophagosomal LC3-II and autophagy adaptor/substrate p62 levels. (A-E) Primary cortical rat neurons were exposed to 3MA (10 mM), E64 (100 µM), or pepstatin A (100 µM) for 24 h. Levels of p62, LC3-I and LC3-II were measured by western blot analysis. (F-J) Primary cortical rat neurons exposed to 40 µM chloroquine (CQ) for 4 h and then analyzed by western blot for p62, LC3-I and LC3-II. *p < 0.05 compared to control, n = 3.