Fig. 5.

Quantification of MitoTracker and LysoTracker co-localization and LC3 puncta in the presence and absence of chloroquine (CQ). (A) Cells were exposed to vehicle versus 40 µM CQ for 4 h and probed with LysoTracker (green) and MitoTracker (red) then live cell imaged at 63×. (B) The co-localization was quantified as the percentage of red signal co-localized with the green signal per field. Data = mean ± SEM, n = 3. (C) Representative immunocytochemistry images of neurons plated at 250,000 per well on autoclaved glass coverslips and treated with either control or CQ, then probed for LC3 puncta. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).