Fig. 4.

TWF9 is specific to pathological Aβ and phosphorylated tau present in AD. a Saturated binding curve in direct ELISA measuring binding affinity of TWF9 to Aβ oligomers. Sandwich ELISA measuring different concentrations of TWF9 and isotype control antibody affinity for b oligomeric Aβ42 and c monomeric Aβ42. Plates were coated with rabbit capture antibody specific to the C-terminus of Aβ42. d PHF1 antibody specific for paired helical filaments containing phosphorylated tau (pS396/pS404) and 468/6E10 antibodies specific for Aβ on an immunoblot of three Alzheimer’s disease (AD) and three nondemented age-matched control human frontal fresh frozen cortex cases used in immunoprecipitation (IP) studies. Fast green reversible protein stain is shown to assess equal loading. e IP product was analyzed by denaturing SDS-PAGE. Quantification of IP results for f Aβ (two-tailed t test: **p < 0.01) and g phosphorylated tau (two-tailed t test: **p < 0.01). For IP experiments, normalized values are shown as the difference between TWF9 and isotype control IgG mean gray values. Data represent the mean ± SD. A.U. arbitrary units, Ctrl control, OD optical density