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. 2018 Jun 18;18:188. doi: 10.1186/s12906-018-2255-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The effects of Zyflamend and an inhibitor of DAPK on the phosphorylation of CaMKK2 (at Ser511), in CWR22Rv1 cells. A Western blot of pCaMKK2 ± Zyflamend (200 μg/mL, 30 min) and following treatment of DAPKi (20 μM, 1 h – 24 h) followed by the treatment of Zyflamend (200 μg/mL, 30 min). B, C Western blot and graph comparison of pCaMKK2 following treatment of DAPKi (20 μM, 24 h) ± Zyflamend (200 μg/mL, 30 min). (D, E) Western blot and graph comparison of pAMPK following treatment of Zyflamend (200 μg/mL, 30 min) and DAPKi (20 μM, 24 h) ± Zyflamend (200 μg/mL, 30 min). Data are presented as mean ± SEM, n = 3. Bars with different letters are statistically different at p < 0.05. Abbreviations: CON, Control; DAPKi, DAPK inhibitor; Zyf, Zyflamend