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. 2018 Jun 18;11:350. doi: 10.1186/s13071-018-2914-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The specificity of the gltA TaqMan primers were validated in silico by assessing the primer sequence conservation with respect to the multiple alignment of available target sequences. Orange outlined box identifies Ehrlichia canis sequences only