Table 3.
Comparison of PCRun® Canine Ehrlichia Kit to the E. canis gltA TaqMan PCR. Totals of 33 samples from the UK and 215 samples from Israel were assessed in a double-blind study, with statistical analysis performed using MedCalc Software [30] (see Additional file 2: Table S1 and Additional file 3: Table S2 for positivity data)
| UK | Israel | |
|---|---|---|
| Benchmark | PCRun (16S) | PCRun (16S) |
| Test assay | Taqman (gltA) | Taqman (gltA) |
| Total tests | 33 | 215 |
| Sensitivity (%) | 100 | 100 |
| Specificity (%) | 100 | 100 |
| Accuracy (%) | 100 | 100 |
| Positive predictive value (%) | 100 | 100 |
| Negative predictive value (%) | 100 | 100 |
| Prevalence (%) | 42 | 21 |
| False discovery rate | 0 | 0 |
| False positive rate | 0 | 0 |
| False omission rate | 0 | 0 |
| False negative rate | 0 | 0 |
| Positive likelihood ratio | 100 | 100 |
| Negative likelihood ratio | 0 | 0 |