Fig. 7.

Potentiating effect of β-eudesmol on suppression Bcl-2 and Bax protein expression by 5-FU and DOX on CCA cells. a-b KKU-100 cells were exposed to β-eudesmol (30 μM), 5-FU (30 μM), DOX (0.1 μM), and combination of β-eudesmol (30 μM) with 5-FU (30 μM) or DOX (0.1 μM) for 24 h. a Effect of β-eudesmol on potentiation of 5-FU and DOX sensitivity on NQO1 enzyme activity analyzed by enzymatic method. b Equal amounts of total protein were examined by Western blot analysis, with appropriate antibodies. β-actin was used as a loading control. Relative band intensities of NQO1, Bcl-2, and Bax were shown. Data are presented as mean ± SD of three independent experiments, triplicate each. * indicates statistically significant difference with each drug alone (paired t-test, p < 0.05). β-Eu= β-Eudesmol, 5-FU= 5-Fluorouracil, DOX= Doxorubicin