Table 3.
Specificity | Clone name | Fluorchrome | Concentration | Dilutiona | Supplier |
---|---|---|---|---|---|
CD3 | 17A2 | Pacific blue | 0.5 mg/ml | 80× | Biolegend |
CD8 | 53–6.7 | A700 | 0.5 mg/ml | 50× | Biolegend |
CD4 | GK1.5 | PE-Cy7 | 0.2 mg/ml | 100× | Biolegend |
CD69 | H1.2F3 | FitC | 0.5 mg/ml | 25× | Biolegend |
α4β7 | DATK32 | APC | 0.2 mg/ml | 25× | Biolegend |
CD62L | MEL-14 | Biotin | 0.5 mg/ml | 200× | Biolegend |
Streptavidin-Pacific Orange | 1 mg/ml | 100× | ThermoFisher | ||
CD44 | IM7 | APC-Cy7 | 0.2 mg/ml | 100× | Biolegend |
aDilution used in a total volume of 25 μl supplemented with PBS + 10% FCS