Table 3.

Antibody specifications

Specificity	Clone name	Fluorchrome	Concentration	Dilutiona	Supplier
CD3	17A2	Pacific blue	0.5 mg/ml	80×	Biolegend
CD8	53–6.7	A700	0.5 mg/ml	50×	Biolegend
CD4	GK1.5	PE-Cy7	0.2 mg/ml	100×	Biolegend
CD69	H1.2F3	FitC	0.5 mg/ml	25×	Biolegend
α4β7	DATK32	APC	0.2 mg/ml	25×	Biolegend
CD62L	MEL-14	Biotin	0.5 mg/ml	200×	Biolegend
		Streptavidin-Pacific Orange	1 mg/ml	100×	ThermoFisher
CD44	IM7	APC-Cy7	0.2 mg/ml	100×	Biolegend

^aDilution used in a total volume of 25 μl supplemented with PBS + 10% FCS